摩登7平台注册体育真人

Rabbit recombinant (RR684) to COX IV

规格或纯度: Recombinant (RR684); Rabbit anti Human COX IV Antibody; WB, IHC, Flow, IF, ICC, IP; Unconjugated

应用: IP,WB,IHC,Flow,IF/ICC,IF/ICC
种属反应性: Human
亚型: Rabbit IgG

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货号 (SKU)	包装规格	是否现货
Ab097410-50μl	50μL	现货
Ab097410-100μl	100μL	现货
Ab097410-1ml	1ml	期货

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图片

Western Blot: 摩登7平台注册体育真人 (Ab097410)

摩登7平台注册体育真人 (Ab097410) - Western Blot
All lanes: 摩登7平台注册体育真人 (Ab097410) at 1/5000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 17 kDa
Observed band size: 18 kDa
Exposure time: 15.1 s

IF: 摩登7平台注册体育真人 (Ab097410)

摩登7平台注册体育真人 (Ab097410) - IF
IF analysis of COX IV (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained 摩登7平台注册体育真人 (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

IHC: 摩登7平台注册体育真人 (Ab097410)

摩登7平台注册体育真人 (Ab097410) - IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of liver tissue labelling COX IV with 摩登7平台注册体育真人 (Ab097410) at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Flow Cytometry: 摩登7平台注册体育真人 (Ab097410)

摩登7平台注册体育真人 (Ab097410) - Flow Cytometry
Overlay histogram showing HeLa cells stained with 摩登7平台注册体育真人 (Ab097410)(Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in 摩登7平台注册体育真人 (Ab097410) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.

IP: 摩登7平台注册体育真人 (Ab097410)

摩登7平台注册体育真人 (Ab097410) - IP
COX IV was immunoprecipitated from 0.4 mg of HeLa whole cell lysate with 摩登7平台注册体育真人 (Ab097410) at 1/50 dilution.
2nd Ab: GAR HRP for IP 1/500

Lane 1: 摩登7平台注册体育真人 (Ab097410) IP in HeLa whole cell lysate
Lane 2: PBS instead of 摩登7平台注册体育真人 (Ab097410) in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 μg (input).

Exposure: 20.0 s

IF: 摩登7平台注册体育真人 (Ab097410)

摩登7平台注册体育真人 (Ab097410) - IF
IF analysis of COX IV (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with 摩登7平台注册体育真人 (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (TRITC) (Ab176448) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

IF: 摩登7平台注册体育真人 (Ab097410)

摩登7平台注册体育真人 (Ab097410) - IF
IF analysis of COX IV (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with 摩登7平台注册体育真人 (Ab097410) at 1/500 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ab176446) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.