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摩登7娱乐注册最新网址 (Ab170151) - IF
IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Antibody (Ab091004) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with 摩登7娱乐注册最新网址 (Ab170151) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.